Research Article| Volume 27, ISSUE 1, P57-64, February 2011

Download started.


Nutrient variability for distillers grains plus solubles and dry matter determination of ethanol by-products1

      This paper is only available as a PDF. To read, Please Download here.


      Three experiments were conducted to evaluate nutrient content and DM determination methods of dry milling byproducts. In Exp. 1, nutrient composition was determined for wet distillers grains plus solubles (WDGS) and modified distillers grains plus solubles (MDGS) from 6 ethanol plants with 10 samples collected per day, across 5 d, and sampling was repeated over 4 separate months. Mean composition was 31.0% CP, 11.9% fat, 0.84% P, and 0.77% S (DM basis). Coefficients of variation for DM content were greater for some plants than others, and variation occurred within and across days. Variability was small for CP and P, whereas fat differed among ethanol plants. Large variation in means and CV were observed for S in period 1, but variation subsequently decreased. Coefficients of variation for S were similar for samples collected within the same day and across days. In Exp. 2, samples of WDGS, MDGS, Dakota Bran Cake, and distillers solubles were used to determine DM content by drying samples at 105°C for 3, 8, and 24 h and 60°C for 24 and 48 h, vacuum oven drying, toluene distillation, and Karl Fischer titration. Compared with toluene distillation, drying at 105°C resulted in less DM (P ≤ 0.10) and vacuum drying and Karl Fischer titration resulted in greater DM (P < 0.01). In Exp. 3, additional WDGS, MDGS, and wet grains with no solubles were used to determine DM with oven drying at 60°C for 48 h, oven drying at 105°C for 3 h, or toluene distillation. Drying at 60°C for 48 h was similar to toluene distillation (P ≥ 0.60).

      Key words

      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'


      Subscribe to
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect


        • Akayezu J.M.
        • Linn J.G.
        • Harty S.R.
        • Cassady J.M.
        Use of distillers grains and co-products in ruminant diets.
        59th Minnesota Nutr. Conf. 1998: 1 (Bloomington, MN)
        • American Feed Industry Association
        Evaluation of analytical methods for analysis of dried distillers grains with solubles.
        (Accessed March 11, 2010.)
        • AOAC
        Official Methods of Analysis.
        16th ed. AOAC, Arlington, VA1999
        • AOCS
        Official Methods and Recommended Practices of the AOCS.
        5th ed. Am. Oil Chem. Soc, Champaign, IL1998
        • Babcock B.A.
        • Hayes D.J.
        • Lawrence J.D.
        Using Distillers Grains in the U.S. and International Livestock and Poultry Industries.
        Midwest Agribusiness Trade Research and Information Center. Iowa State University, Ames, IA2008
        • Brahmakshatriya R.D.
        • Donker J.D.
        Five methods for determination of silage dry matter.
        J. Dairy Sci. 1971; 54: 1470
        • Erwin E.S.
        • Marco G.J.
        • Emery E.M.
        Volatile fatty acid analyses of blood and rumen fluid by gas chromatography.
        J. Dairy Sci. 1961; 44: 1768
        • Holt S.M.
        • Pritchard R.H.
        Composition and nutritive value of corn co-products from dry milling ethanol plants.
        South Dakota Beef Rep. 2004; 2004–01: 1-7
        • Kaiser R.M.
        Variation in composition of distillers wet grains with solubles.
        4-State Dairy Nutr. Manage. Conf. Midwest Plan Service, Ames, IA2005: 191
        • Mo M.
        • Tjornhom T.
        Losses of carbon-containing substances during dry matter determination by oven drying.
        Acta Agric. Scand. 1978; 18: 196
        • NRC
        Nutrient Requirements of Beef Cattle.
        Press, Washington, DC, Natl. Acad1996
        • Spiehs M.J.
        • Whitney M.H.
        • Shurson G.C.
        Nutrient database for distillers dried grains with solubles produced from new ethanol plants in Minnesota and South Dakota.
        J. Anim. Sci. 2002; 80: 2639
        • Thiex N.
        • Van Erem T.
        Comparisons of Karl Fischer method with oven methods for determination of water in forages and animal feeds.
        J. AOAC. 1999; 82: 799
        • Thiex N.
        • Van Erem T.
        Determination of water (moisture) and dry matter in animal feed, grain and forage (plant tissue) by Karl Fischer titration: Collaborative study.
        J. AOAC Int. 2002; 85: 318
        • Tinsdale S.L.
        • Nelson W.L.
        • Beaton J.D.
        Soil Fertility and Fertilizers.
        4th ed. Macmillan Publ. Co., New York, NY1985
        • Vander Pol K.J.
        • Luebbe M.K.
        • Crawford G.I.
        • Erickson G.E.
        • Klop-fenstein T.J.
        Performance and digestibility characteristics of finishing diets containing distillers grains, composites of corn processing coproducts, or supplemental corn oil.
        J. Anim. Sci. 2009; 87: 639