ABSTRACT
In previous studies using Ankom GP vented in vitro systems (Ankom Technology, Macedon,
NY), methane production was estimated from gas production (GP) and a single gas sample
from the incubation vessel’s headspace at the end of the incubation. An accurate method
requires measurement of methane in the incubation vessel’s headspace and in the gases
vented over the incubation period. This research aimed to determine whether the act
of collecting the gas vented from the Ankom GP system influenced the estimation by
the Ankom GP system of the volume of gas produced or the composition of the gas produced.
A method involving collecting gas through a long (304 cm), narrow (1 mm i.d.) gas
line estimated greater GP than a control method not involving collection of gas. A
method involving collecting gas through a short (22 cm), wide (4 mm i.d.) gas line
did not affect headspace methane percentage or estimates of GP. It is concluded that
in vitro methane production can be accurately estimated by using the Ankom GP system
together with collection of vented gases into gas collection bags, but only if the
Ankom GP system is connected to the gas collection bag via a short, wide gas line.
Key words
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Article info
Publication history
Accepted:
May 30,
2018
Received:
March 26,
2018
Footnotes
The authors declare no conflict of interest.
Identification
Copyright
Copyright © 2018, American Registry of Professional Animal Scientists. All rights reserved.
