ABSTRACT
Objective
Phytase enzymes support broiler phosphorus requirements and vary in thermal stability
and manufacturer suggested protocols for determining activity. Our objective was to
evaluate nutrient degradation, determined using phytase markers, broiler performance,
and tibia mineralization from diets based on corn and soybean meal subjected to increasing
steam conditioning temperatures.
Materials and Methods
Three commercial phytase products (B Trich, E Pichia, E Trich) were analyzed for activity
at 2 laboratories. The lower obtained activity was used to include each product at
1,000 phytase units/kg of the diet. Each product was applied to a negative control
(NC) diet before steam conditioning at 82, 88, or 93°C, pelleting, and crumbling.
A 4 × 3 factorial arrangement in a randomized complete block design created 12 treatments
fed to 8 replicate cages of 10 Hubbard × Cobb straight-run broilers for 20 d.
Results and Discussion
Phytase product effects demonstrated that NC-fed broilers had the lowest feed intake
and live weight gain, whereas broilers fed E Trich had the greatest feed intake and
live weight gain (P < 0.05). Broilers fed B Trich and E Pichia had lower feed conversion ratio compared
with NC-fed broilers, and broilers fed E Trich were intermediate (P < 0.05). Increased conditioning temperature sequentially decreased feed intake and
live weight gain (P < 0.05). Main effects interacted such that tibia ash measures decreased as conditioning
temperature increased; however, mineralization differences were less dramatic in NC-fed
birds.
Implications and Applications
Increased conditioning temperature may have decreased nutrient availability per se
and denatured phytase products, or both factors may have contributed to performance
detriment.
Key words
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LITERATURE CITED
- Does supplemental dietary microbial phytase improve amino acid utilization? A perspective that it does not..J. Anim. Sci. 2003; 81: 78-85
- Phytic acid chemistry: Influence of phytin-phosphorus availability and phytase efficacy..J. Appl. Poult. Res. 2002; 11: 471-480
- Official Methods of Analysis of AOAC International.17th ed. AOAC Int, Arlington, VA2000
- Exogenous enzymes in monogastric nutrition—Their current value and future benefits..Anim. Feed Sci. Technol. 2000; 86: 1-13
- Exogenous enzymes for pigs and poultry..19087461Nutr. Res. Rev. 1998; 11: 91-114
- The effects of steam conditioning and antimicrobial inclusion on feed manufacturing and inactivation of Enterococcus faecium, a Salmonella surrogate..J. Appl. Poult. Res. 2018; 27: 472-482
- The effects of Spirulina algae inclusion and conditioning temperature on feed manufacture, pellet quality, and true amino acid digestibility..Anim. Feed Sci. Technol. 2017; 224: 20-29
- The effect of steam conditioning practices on pellet quality and growing broiler nutritional value..J. Appl. Poult. Res. 2008; 17: 249-261
- Total phosphorus, phytate-phosphorus, and phytase activity in plant feedstuffs..Anim. Feed Sci. Technol. 1994; 47: 19-29
- Increasing mixer-added fat reduces the electrical energy required for pelleting and improves exogenous enzyme efficacy for broilers..J. Appl. Poult. Res. 2011; 20: 75-89
- Effect of phytase supplementation on performance, tibia ash, and serum P in broilers fed diets with different levels of phosphorus..J. Appl. Anim. Res. 2009; 36: 137-140
- Phytic acid complex in feed ingredients.in: Coelho M.B. Kornegay E.T. Pages 69–75 in Phytase in Animal Nutrition and Waste Management. BASF Ref. Man., BASF Corp., Mount Olive, NJ1999
- Enzyme Nomenclature: Recommendation of the Nomenclature Committee of the International Union of Biochemistry.Acad. Press, New York, NY1979
- Effect of pelleting mixed feeds on phytase activity and the apparent absorbability of phosphorus and calcium in pigs..Anim. Feed Sci. Technol. 1990; 28: 233-242
- Effects of varying conditioning temperature and mixer-added fat on feed manufacturing efficiency, 28- to 42-day broiler performance, early skeletal effect, and true amino acid digestibility..J. Appl. Poult. Res. 2014; 23: 444-455
- The phytase analytical activity of pelleted diets may not adequately describe efficacy in the bird..J. Appl. Poult. Res. 2012; 21: 492-501
- Evaluation of increasing levels of a microbial phytase in phosphorus deficient broiler diets via live broiler performance, tibia bone ash, apparent metabolizable energy, and amino acid digestibility..27444440Poult. Sci. 2017; 96: 370-382
- Phytates: Occurrence, bioavailability, and implications in poultry nutrition..Poult. Avian Biol. Rev. 1995; 6: 125-143
- Effect of mixing and sampling method on pelleted feed nutrient analysis and diet formulation validation..J. Appl. Poult. Res. 2016; 26: 219-225
- Effects of modifying diet and feed manufacture concern areas that are notorious for decreasing pellet quality..J. Appl. Poult. Res. 2018; 0: 1-9
- Microbial phytase in poultry nutrition..Anim. Feed Sci. Technol. 2007; 135: 1-41
- Physical quality of pelleted animal feed. 1. Criteria for pellet quality..Anim. Feed Sci. Technol. 1996; 61: 89-112
Article info
Publication history
Accepted:
March 5,
2019
Received:
January 18,
2019
Footnotes
The authors declare no conflict of interest.
Identification
Copyright
© 2019 American Registry of Professional Animal Scientists. All rights reserved. All rights reserved.
