ABSTRACT
Objective
The objective of this experiment was to determine the effects of increasing doses
of a seaweed extract (Ecklonia radiata; SWE) and SWE with quebracho (Schinopsis quebrachocolorado) tannin extract (TE) on in vitro fermentation.
Materials and Methods
Two in vitro experiments were conducted using the Daisy II incubator, where repeated
measurements were taken. In Exp. 1, treatments were increasing levels (0, 2, 9.5,
or 31.5 μL per fermentation jar) of the SWE, and in Exp. 2, treatments were either
no SWE or TE (CON), the optimal dose (the regression minimum) determined from Exp.
1 of SWE only (SWO), 4 mg of TE and SWO (LTS), or 10 mg of TE and SWO (HTS).
Results and Discussion
There were no time-by-dose interactions (P ≥ 0.13) in either experiment. Only the NH3 from the 2-μL dose was different (P = 0.04) from the 0-μL dose. No other differences (P > 0.10) were detected in Exp. 1. A mixed model regression determined a cubic relationship
between dose and NH3 (P = 0.02), with the minimum at 5 μL. In Exp. 2, SWO reduced (P = 0.02) NH3 compared with all other treatments. The SWO treatment also reduced total VFA compared
with the other treatments (P = 0.01). The LTS and HTS were not different from the CON for any variables (P > 0.1).
Implications and Applications
Under the context of this experiment, we conclude that low levels of SWE reduce ruminal
NH3 and suggest that urinary N may be reduced in vivo, but this was negated in the presence
of condensed tannins.
Key words
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Article info
Publication history
Accepted:
August 8,
2019
Received:
June 19,
2019
Footnotes
The authors declare no conflict of interest.
Identification
Copyright
© 2019 American Registry of Professional Animal Scientists. Published by Elsevier Inc. All rights reserved.
