ABSTRACT
Objective
The objectives of this study were (1) to determine the effects of 3 levels of a rumen-protected
Lys (RP-Lys) prototype on milk production in dairy cows fed a diet deficient in MP-Lys
and (2) to assess the effects of treatments using AMTS.Cattle.Professional (AMTS =
Agricultural Modeling and Training System; Groton, NY).
Materials and Methods
Ten multiparous, Holstein cows were used in a 5 × 10 Latin rectangle (rows = five
18-d periods, columns = 10 cows). Treatments were (1) control (0 g of RP-Lys), (2)
30 g of Lys supplemented from RP-Lys using Aji-Pro L (APL; Ajinomoto, Chicago, IL),
(3) 17.5 g of Lys supplemented from RP-Lys prototype (RPLL), (4) 21.8 g of Lys supplemented
from RP-Lys prototype (RPLM), and (5) 31.5 g of Lys supplemented from RP-Lys prototype
(RPLH). The basal diet was formulated with a MP-Lys balance of −16.5 g/d. Milk and
blood samples were analyzed for milk components and plasma AA concentrations. Treatments
were assessed for MP-Lys balance using actual chemical composition of feed ingredients,
DMI, and milk production using the AMTS.
Results and Discussion
Regression analysis revealed that RP-Lys prototype supplementation increased milk
and lactose yields linearly (P = 0.03 and 0.04). There was a trend for RP-Lys prototype to increase protein percent
quadratically (P = 0.07). Treatments did not affect plasma Lys concentrations. The retrospective evaluation
of treatments showed an MP-Lys balance of −17 g/d for control and −2, −7, and 8 g/d
for RPLL, RPLM, and RPLH treatments, respectively. Collectively, these results indicated
that the RP-Lys prototype delivered Lys to support milk production.
Implications and Applications
Results indicate that supplementation with an RP-Lys prototype increased milk production
in cows fed a diet deficient in RP-Lys. These results may benefit dairy cows fed corn-based
diets.
Key words
To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe toAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
LITERATURE CITED
- Official Methods of Analysis.16th ed. AOAC Int, Gaithersburg, MD1999
- Official Methods of Analysis.17th ed. AOAC Int., Gaithersburg, MD2000
- Effects of reduced dietary protein and supplemental rumen-protected essential amino acids on the nitrogen efficiency of dairy cows.J. Dairy Sci. 2014; 97 (25022689): 5688-5699
- Effect of ruminally protected methionine on splanchnic metabolism of amino acids in lactating dairy cows.J. Dairy Sci. 2006; 89 (16606732): 1621-1634
- Effect of whole cottonseed, gossypol, and ruminally protected lysine supplementation on milk yield and composition.J. Dairy Sci. 1997; 80 (9241597): 1358-1365
- Assessing bioavailability of ruminally protected methionine and lysine prototypes.J. Dairy Sci. 2019; 102 (30852023): 4014-4024
- Determination of starch, including maltooligosaccharides, in animal feeds: Comparison of methods and a method recommended for AOAC collaborative study.J. AOAC Int. 2009; 92 (19382561): 42-49
- Milk production of dairy cows fed differing concentrations of rumen-degraded protein.J. Dairy Sci. 2006; 89 (16357288): 249-259
- Jugular-infused methionine, lysine and branched-chain amino acids does not improve milk production in Holstein cows experiencing heat stress.Animal. 2017; 11 (28514978): 2220-2228
- Lowering rumen-degradable protein maintained energy-corrected milk yield and improved nitrogen-use efficiency in multiparous lactating dairy cows exposed to heat stress.J. Dairy Sci. 2017; 100 (28755937): 8132-8145
- Lowering rumen-degradable and rumen-undegradable protein improved amino acid metabolism and energy utilization in lactating dairy cows exposed to heat stress.J. Dairy Sci. 2018; 101 (29128223): 386-395
- Effect of dietary protein level and rumen-protected amino acid supplementation on amino acid utilization for milk protein in lactating dairy cows.J. Dairy Sci. 2015; 98 (25547302): 1885-1902
- Effect of two levels of crude protein and methionine supplementation on performance of dairy cows.J. Dairy Sci. 2003; 86 (14740841): 4033-4042
- Effects of dietary crude protein and rumen-degradable protein concentrations on urea recycling, nitrogen balance, omasal nutrient flow, and milk production in dairy cows.J. Dairy Sci. 2016; 99 (27236760): 6298-6310
- Productive responses of lactating dairy cattle to supplementing high levels of ruminally protected lysine using a rumen protection technology.Anim. Feed Sci. Technol. 2011; 168: 30-41
- Amino acid limitation and flow to duodenum at four stages of lactation. 1. Sequence of lysine and methionine limitation.J. Dairy Sci. 1992; 75 (1474215): 3486-3502
- Nitrogen requirements of cattle.in: Nitrogen and Phosphorus Nutrition of Cattle and Environment. CAB Int., Wallingford, UK2005: 13-70
- Prediction of the energy value of cow's milk.J. Dairy Sci. 1965; 48 (5843077): 1215-1223
- Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition.J. Dairy Sci. 1991; 74 (1660498): 3583-3597
- Effects of dietary supplementation of methionine and lysine on milk production and nitrogen utilization in dairy cows.J. Dairy Sci. 2010; 93 (20655436): 3661-3670
- The plasma free amino acid dose-response technique: A proposed methodology for determining lysine relative bioavailability of rumen-protected lysine supplements.J. Dairy Sci. 2017; 100 (28964520): 9585-9601
Article info
Publication history
Accepted:
July 21,
2019
Received:
May 30,
2019
Footnotes
Three of the authors work for Balchem Corporation, which provided funding for this research. The other authors declare no conflict of interest.
Identification
Copyright
© 2019 American Registry of Professional Animal Scientists. Published by Elsevier Inc. All rights reserved.