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FORAGES AND FEEDS: Original Research| Volume 36, ISSUE 5, P583-591, October 2020

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Performance and income over feed costs when feeding alfalfa or grass hays and corn or wheat grains to high-producing dairy cows

      ABSTRACT

      Objective

      The objective of this study was to evaluate the production performance, nutrient digestibility, and income over feed cost (IOFC) of high-producing dairy cows consuming diets containing alfalfa or grass hays with either corn or wheat grain.

      Materials and Methods

      Twenty-four Holstein cows were randomly assigned to 1 of 4 diets in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments (hay and grain types) and 21-d periods. Diets were formulated using a least-cost approach. To determine revenues from milk produced, the amount of ECM (kg·d−1) was multiplied by $0.303·kg−1 (i.e., class III milk price; US Federal Milk Marketing Order 5). The cost of the ration provided by the formulation software ($·cow−1·d−1) was divided by the predicted DMI (kg·cow−1·d−1) to obtain the cost of feed ($·kg−1), which was then multiplied by DMI (kg·cow−1·d−1) to provide the actual daily feed cost ($·cow−1·d−1).

      Results and Discussion

      Cows consuming diets containing alfalfa hay consumed more DM than cows consuming diets with grass hay (27.1 vs. 24.4 kg·d−1). Cows consuming diets containing alfalfa hay produced more milk than cows consuming diets containing grass hay (47.5 vs. 44.7 kg·d−1). Milk from cows consuming diets containing grass hay had greater fat concentrations than milk from cows consuming diets containing alfalfa hay (4.22 vs. 3.89%). Using hay prices of $418 and $154∙t−1, respectively, for alfalfa and grass hays, diets containing grass hay resulted in greater IOFC than diets containing alfalfa hay ($8.39·d−1 vs. $7.68·d−1, respectively).

      Implications and Applications

      Results of this study showed that IOFC can be supported when feeding grass hay using a least-cost ration formulation approach.

      Key words

      INTRODUCTION

      The chronic low-milk-price scenario that occurred from 2015 to 2018 substantially reduced revenues and negatively affected the profitability of US dairy farms. This unfavorable scenario obligated farmers to maximize income over feed costs (IOFC) by increasing milk production, reducing feeding costs, or both. As feed costs can be directly related to milk production but milk production may not be related to profitability (

      Ferreira, G. 2015. Income Over Feed Costs in the Dairy Enterprise. Virginia Cooperative Extension, DASC-51P. Virginia Polytech. Inst. State Univ., Blacksburg, VA.

      ), seeking maximum milk production can become a double-edged sword if accomplished at the expense of increasing feed costs.
      Managers or nutritionists have indirect control of milk production. In this regard, maximizing IOFC by increasing milk production depends on multiple biological and environmental factors, such as cows’ responses to nutritional management, herd health and fertility, and cow comfort. Conversely, managers or nutritionists have some direct control of feeding costs. Therefore, maximizing IOFC by reducing feeding costs depends on a few managerial decisions, such as selecting less expensive ingredients and formulating diets that meet production requirements. Under the scope that cows require nutrients and not ingredients (

      St-Pierre, N. R. 2003. Cost of Nutrients in Feedstuffs. Buckeye Dairy News. Vol. 5, No. 2. Accessed Feb. 26, 2020. https://dairy.osu.edu/newsletter/buckeye-dairy-news/volume-5-issue-2/cost-nutrients-feedstuffs.

      ), using a least-cost formulation approach, while meeting performance requirements or mitigating environmental impacts, may help minimize feeding costs and support IOFC by selecting less expensive ingredients (
      • Saddoris-Clemons K.
      • Schneider J.
      • Feoli C.
      • Cook D.
      • Newton B.
      Cost-effective feeding strategies for grow-finish pigs..
      ;
      • Mackenzie S.G.
      • Leinonen I.
      • Ferguson N.
      • Kyriazakis I.
      Towards a methodology to formulate sustainable diets for livestock: accounting for environmental impact in diet formulation..
      ).
      When feeding high-producing dairy cows, including sufficient physically effective NDF from hay in the ration is a good strategy to ensure rumen health, optimize nutrient utilization, and increase milk fat concentration (
      • Mertens D.R.
      Creating a system for meeting the fiber requirements of dairy cows..
      ;
      • Zebeli Q.
      • Tafaj M.
      • Steingass H.
      • Metzler B.
      • Drochner W.
      Effects of physically effective fiber on digestive processes and milk fat content in early lactating dairy cows fed total mixed rations..
      ;
      • Kammes K.L.
      • Allen M.S.
      Nutrient demand interacts with grass particle length to affect digestion responses and chewing activity in dairy cows..
      ). In the mid-Atlantic region of the United States, where the availability of alfalfa hay is limited, nutritionists and managers may be obligated or tempted to buy expensive alfalfa hay from far-away regions (e.g., Great Plains or Midwest), to exclude alfalfa hay from the diet, or to include locally grown hay (e.g., grass hay) of poorer quality than alfalfa hay in the diet. Even though the latter alternative seems unfavorable when comparing hay quality on a DM basis, including grass hay in the ration may still be a less expensive alternative when considering specific nutrients (e.g., physically effective NDF).
      In most parts of the United States, milk price is determined, at least, by the concentration of fat in milk. Therefore, dietary factors affecting milk fat concentration may affect IOFC. Differences in ruminal starch digestibility among cereal grains can affect milk fat concentrations. For example, lower milk fat concentrations have been reported when wheat grain replaced corn grain in diets fed to dairy cattle (
      • Gozho G.N.
      • Mutsvangwa T.
      Influence of carbohydrate source on ruminal fermentation characteristics, performance, and microbial protein synthesis in dairy cows..
      ;
      • Moate P.J.
      • Jacobs J.L.
      • Hannah M.C.
      • Morris G.L.
      • Beauchemin K.A.
      • Alvarez Hess P.S.
      • Eckard R.J.
      • Liu Z.
      • Rochfort S.
      • Wales W.J.
      • Williams S.R.O.
      Adaptation responses in milk fat yield and methane emissions of dairy cows when wheat was included in their diet for 16 weeks..
      ), although differences in ECM have not always been observed (
      • Moate P.J.
      • Jacobs J.L.
      • Hannah M.C.
      • Morris G.L.
      • Beauchemin K.A.
      • Alvarez Hess P.S.
      • Eckard R.J.
      • Liu Z.
      • Rochfort S.
      • Wales W.J.
      • Williams S.R.O.
      Adaptation responses in milk fat yield and methane emissions of dairy cows when wheat was included in their diet for 16 weeks..
      ). As physically effective NDF is necessary to support milk fat concentration (
      • Mertens D.R.
      Creating a system for meeting the fiber requirements of dairy cows..
      ), cows consuming diets with wheat grain as a primary starch source might be more sensitive to replacement of alfalfa hay with grass hay than cows consuming diets with corn grain as a primary starch source. If such an interaction exists, then an interaction in IOFC might also be observed.
      Relative to feeding diets including some alfalfa hay, we hypothesized that production performance can be supported when feeding diets including grass hay instead. We also hypothesized that this practice can support IOFC. To be more comprehensive, we tested these hypotheses using wheat or corn as grain sources. Therefore, the objective of this study was to evaluate the production performance, the nutrient digestibility, and the IOFC of high-producing dairy cows consuming diets containing either alfalfa or grass hays with corn or wheat grains.

      MATERIALS AND METHODS

      Experimental Design

      All procedures involving animals were approved by the Institutional Animal Care and Use Committee of Virginia Tech. Eight primiparous (572 ± 33 kg of BW and 50 ± 16 DIM at the beginning of the experiment) and 16 multiparous (674 ± 43 kg of BW and 52 ± 18 DIM at the beginning of the experiment) Holstein cows were randomly assigned to 1 of 4 diets (Table 1) in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments and 21-d periods. The first 2 wk of each period were used for rumen adaptation to the diets, and the average milk production and DMI data of wk 3 were used for statistical analysis. Cows were assigned to squares based on parity (1, 2, and ≥3) and milk production (10-d pretrial period), housed in a 24-stall pen within a freestall barn, and fed once daily (1100 h) using a Calan gate system (American Calan Inc., Northwood, NH). Cows were trained for 2 wk before the beginning of the experiment to locate their assigned doors. Dietary treatments were offered to individual cows as TMR.
      Table 1Ingredient and chemical composition of experimental diets
      Diets contained either alfalfa hay or grass hay with either corn grain or wheat grain. Nutrient concentrations are based on analyses of feed ingredients (n = 4).
      ItemAlfalfa hayGrass hay
      Corn grainWheat grainCorn grainWheat grain
      Ingredient, % DM
       Corn silage36.935.933.131.6
       Alfalfa hay14.114.1
       Grass hay19.618.7
       Corn grain17.521.2
       Wheat grain16.923.2
       Soybean meal19.57.021.817.5
       Soybean hulls8.83.8
       Corn distillers grains18.15.0
       Calcium salts of fatty acids
      Calcium salts of fatty acids (Virtus Nutrition LLC, Corcoran, CA).
      0.91610.91650.95450.9115
       Sodium bicarbonate1.09931.09981.14551.0938
       Salt0.54960.54990.57270.5469
       Magnesium oxide0.18320.18330.19090.1823
       Bentonite0.91610.91650.95450.9115
       Trace mineral premix
      Contained 22.25% calcium; 7.50% magnesium; 2.75% potassium; 3.90% sulfur; 1.50% manganese; 1.50% zinc; 9,500 mg/kg iron; 2,500 mg/kg copper; 200 mg/kg iodine; 200 mg/kg cobalt; 66 mg/kg selenium; 227,273 IU·kg−1 vitamin A; 136,364 IU·kg−1 vitamin D3; 636 IU·kg−1 vitamin E.
      0.45800.45820.47730.4557
       Vitamin ADE premix
      Contained 3,500 IU·kg−1 vitamin A; 950 IU·kg−1 vitamin D3; 2,000 IU·g−1 vitamin E.
      0.04580.04580.04770.0456
       Vitamin E
      Contained 500 IU·g−1 of premix.
      0.00370.00370.00380.0036
       Rumensin 90
      Contained 200 mg of monensin per gram of product (Elanco Animal Health, Indianapolis, IN).
      0.00690.00690.00720.0068
      Nutrients, % DM
       Ash6.86.96.65.7
       CP15.615.715.114.6
       NDF28.430.330.531.0
       Forage NDF17.217.324.323.2
       Starch23.723.827.027.6
      Cost,
      According to the output from CPM Dairy (CAHP Software Information, Philadelphia, PA).
      $·kg of DM−1
      0.2870.2840.2530.261
      Particle size distribution,
      Particle size distribution determined using a Penn State Particle Separator (Lammers et al., 1996).
      % DM
       >19.0 mm7.19.48.611.2
       8 to 19 mm32.231.526.231.5
       <8 mm60.759.165.157.4
      1 Diets contained either alfalfa hay or grass hay with either corn grain or wheat grain. Nutrient concentrations are based on analyses of feed ingredients (n = 4).
      2 Calcium salts of fatty acids (Virtus Nutrition LLC, Corcoran, CA).
      3 Contained 22.25% calcium; 7.50% magnesium; 2.75% potassium; 3.90% sulfur; 1.50% manganese; 1.50% zinc; 9,500 mg/kg iron; 2,500 mg/kg copper; 200 mg/kg iodine; 200 mg/kg cobalt; 66 mg/kg selenium; 227,273 IU·kg−1 vitamin A; 136,364 IU·kg−1 vitamin D3; 636 IU·kg−1 vitamin E.
      4 Contained 3,500 IU·kg−1 vitamin A; 950 IU·kg−1 vitamin D3; 2,000 IU·g−1 vitamin E.
      5 Contained 500 IU·g−1 of premix.
      6 Contained 200 mg of monensin per gram of product (Elanco Animal Health, Indianapolis, IN).
      7 According to the output from CPM Dairy (CAHP Software Information, Philadelphia, PA).
      8 Particle size distribution determined using a Penn State Particle Separator (
      • Lammers B.P.
      • Buckmaster D.R.
      • Heinrichs A.J.
      A simplified method for the analysis of particle sizes of forage and total mixed rations..
      ).

      Ration Formulation

      Four diets were formulated using a least-cost formulation approach (CPM Dairy 3.0.8.1; CAHP Software Information, Philadelphia, PA). For the ration formulation, we considered the inclusion of either alfalfa hay or grass hay as a forage source and the inclusion of either corn grain or wheat grain as the grain source. Corn silage was the forage in greatest concentration in each dietary treatment.
      Rations were formulated considering a 620-kg lactating cow at 45 DIM of its second lactation and producing 40 kg of milk per day (3.85% fat and 3.05% true protein). Constraints for the least-cost formulation (Table 2) included DMI (kg/d), ME (% required), MP (% required), dietary forage (% DM), NDF (% DM), nonfiber carbohydrates (% DM), and starch (% DM). In addition, additives were set to meet nutrient requirements, set to follow feeding recommendations, or based on personal experience as a nutritionist.
      Table 2Nutrient and ingredient constraints (kg∙d−1 unless noted otherwise) used for ration formulation
      ItemMinimumMaximum
      DMI, % required100100
      ME, % required100105
      MP, % required100110
      Dietary forage, % DM5065
      NDF, % DM3033
      Nonfiber carbohydrates, % DM3045
      Starch, % DM2630
      Calcium salts of fatty acids0.2000.200
      Sodium bicarbonate0.2400.240
      Salt0.1200.120
      Bentonite0.2000.200
      Magnesium oxide0.0400.040
      Trace mineral premix0.1000.100
      Vitamin ADE premix0.0100.010
      Vitamin E0.00080.0008
      Rumensin 90
      Elanco Animal Health (Indianapolis, IN).
      0.00150.0015
      1 Elanco Animal Health (Indianapolis, IN).
      Pelleted concentrates were prepared at a commercial feed mill (Big Spring Mill Inc., Elliston, VA). Therefore, ration formulation was performed considering corn silage, alfalfa or grass hays, and selected ingredients commonly available. The latter group included corn and wheat grains; soybean meal (48% CP); soybean hulls; corn distillers grains with solubles; and all minerals, vitamins, and additives.
      Chemical compositions of corn silage, alfalfa hay, and grass hay were determined in a commercial laboratory (Cumberland Valley Analytical Services, Waynesboro, PA) a priori to the formulation of the diets. The output of these analyses (Table 3) was then transferred to the ration formulation tool. For grains and commodities, chemical compositions from the feed library in the ration formulation tool were used.
      Table 3Chemical composition
      Near-infrared reflectance spectroscopy data from a commercial laboratory (Cumberland Valley Analytical Services, Waynesboro, PA) a priori to formulating the experimental diets.
      (DM basis) of forages used for ration formulation
      ItemCorn silageAlfalfa hayGrass hay
      DM, %36.587.587.5
      CP, %5.918.08.6
      Soluble protein, %3.17.12.1
      NH3-N, %0.70.80.7
      ADICP,
      ADICP = acid detergent insoluble CP.
      %
      0.71.41.6
      NDICP,
      NDICP = neutral detergent insoluble CP.
      %
      0.82.63.7
      ADF, %20.937.245.4
      NDF, %36.644.675.0
      Lignin, %2.57.96.7
      Ash, %2.49.96.0
      Crude fat, %2.82.22.0
      Sugar, %1.04.82.5
      Starch, %38.51.92.1
      1 Near-infrared reflectance spectroscopy data from a commercial laboratory (Cumberland Valley Analytical Services, Waynesboro, PA) a priori to formulating the experimental diets.
      2 ADICP = acid detergent insoluble CP.
      3 NDICP = neutral detergent insoluble CP.

      Prices of Feeds

      All feed prices are reported on an as-fed basis. Alfalfa hay was bought from a hay farm in Kansas at a delivered price of $418·t−1. Grass hay was grown and baled on farm (Blacksburg, VA) and consisted of a mixture of fescue (Festuca arundinacea) and orchardgrass (Dactylis glomerata). Mixed-grass hay was priced at $154·t−1 according to the “Good/Mixed Grass/Large round bale” price at the hay auction (July 18, 2018) in Harrisonburg, Virginia ($84·t−1;

      USDA. 2018. Virginia Hay Report Rushville Semimonthly Hay Auction, Harrisonburg, VA, on Wed. Jul. 18, 2018. Accessed Feb. 26, 2020. https://search.ams.usda.gov/mndms/2018/07/RH_GR31020180719.TXT.

      ) and considering $70·t−1 for hauling and marketing expenses.
      Corn silage was priced ($77·t−1) as the average between the Low and High prices for the Southeast in the Feed column of the Market Watch supplement of

      Progressive Dairyman. 2018. Market watch: Feed. Progr. Dairyman 32:4–5.

      . Soybean meal ($513·t−1), soybean hulls ($195·t−1), and corn distillers grains with solubles ($354·t−1) were priced similarly to corn silage but with an additional $46·t−1 to cover hauling and marketing expenses. Corn ($186·t−1) and wheat ($244·t−1) grains were priced following September 2018 prices (www.cmegroup.com) during August 2018. The latter prices also included hauling and marketing expenses of $46·t−1. As they were included at constant rates, feed additives were not priced for ration formulation.

      Nutrient Digestibility

      Total-tract nutrient digestibility was estimated using lanthanum chloride as an external marker as described by
      • Yang Y.
      • Ferreira G.
      • Teets C.L.
      • Corl B.A.
      • Thomason W.E.
      • Griffey C.A.
      Effects of feeding hull-less barley on production performance, milk fatty acid composition, and nutrient digestibility of lactating dairy cows..
      . To obtain a final dietary lanthanum concentration of 40 mg·kg of DM−1, 42 kg of the marker solution (density = 1.15 g·mL−1; lanthanum concentration = 102 g·L−1) was sprayed onto 1,750 kg of soybean meal that was incorporated into the concentrate pellets. Fecal grab samples were collected for each period across 3 consecutive days (starting on d 17) at 6-h intervals skipping sampling times 2 h at the end of each day. Lanthanum concentration was determined in TMR and fecal samples by inductively coupled plasma atomic emission spectroscopy (Spectro Arcos II ICP-AES, SPECTRO Analytical Instruments GmbH, Kleve, Germany).

      Sample Collection and Analysis

      Feed offered and feed refused was weighed every day to determine DMI of individual cows. Samples of feed offered and refused were collected twice a week (Tuesday and Friday) from the feed bunks of 3 cows of each treatment immediately after feed delivery and before consumption began and composited by period. Samples of corn silage, alfalfa and grass hays, and pelleted concentrates were collected weekly and composited by period.
      All samples were dried to a constant weight at 55°C in a forced-air oven and ground to pass through a 1-mm screen of a Wiley mill (Thomas Scientific, Swedesboro, NJ). Ash concentration was determined after combusting samples in a furnace (Thermolyne 30400, Barnstead International, Dubuque, IA) for 3 h at 600°C (method 942.05,

      AOAC International. 2019. Official Methods of Analysis. 21st ed. AOAC Int., Rockville, MD.

      ). Crude protein concentration was calculated as percent N × 6.25 after combustion analysis (method 990.03,

      AOAC International. 2019. Official Methods of Analysis. 21st ed. AOAC Int., Rockville, MD.

      ) using a Vario El Cube CN analyzer (Elementar Americas Inc., Mount Laurel, NJ). Ash-free NDF and ADF concentrations were determined using the Ankom200 Fiber Analyzer (Ankom Technology, Macedon, NY). Sodium sulfite and α-amylase (Ankom Technology) were included for NDF analysis (
      • Ferreira G.
      • Mertens D.R.
      Measuring detergent fibre and insoluble protein in corn silage using crucibles or filter bags..
      ). Acid detergent fiber and lignin concentrations were determined sequentially. After determining ADF weights, residues were incubated for 3 h in 72% sulfuric acid within a 4-L jar that was placed in a DaisyII Incubator (Ankom Technology). Starch concentration was determined using the acetate buffer method of
      • Hall M.B.
      Determination of starch, including maltooligosaccharides, in animal feeds: Comparison of methods and a method recommended for AOAC collaborative study..
      with α-amylase from Bacillus licheniformis (FAA, Ankom Technology) and amyloglucosidase from Aspergillus niger (E-AMGDF, Megazyme International, Wicklow, Ireland). In addition, TMR samples were collected weekly, and the distribution of particles in the TMR (Table 1) was determined using the Penn State Particle Separator (
      • Lammers B.P.
      • Buckmaster D.R.
      • Heinrichs A.J.
      A simplified method for the analysis of particle sizes of forage and total mixed rations..
      ).
      Milk samples (a.m. and p.m. milkings) were collected on d 16 and 17 of each period for the determination of milk fat, true protein, lactose, and MUN concentrations with a CombiFoss FT+ Fourier transform infrared analyzer (Foss, Hillerød, Denmark) by United DHIA (Radford, VA).
      To determine fiber digestion kinetics (4 samples of each hay type), 0.25 g of hay was put into Ankom F57 porous bags previously rinsed with acetone to perform in vitro digestibility. All bags were incubated in a buffered inoculum for 0, 3, 6, 12, 24, 48, 96, and 240 h using a rotating jar incubation system (DairyII, Ankom Technology). After collecting rumen fluid from 3 cows consuming a diet containing 32% corn silage, 4% alfalfa hay, and 64% concentrate, a composite rumen buffered inoculum was prepared as described by
      • Ferreira G.
      • Mertens D.R.
      Chemical and physical characteristics of corn silages and their effects on in vitro disappearance..
      . In vitro NDF disappearance (IVNDFD) parameters were determined using Proc NLIN of SAS according to Equation [1]:
      IVNDFD(%)=B(%)×(1ekt),
      [1]


      where fraction B is the potentially digestible NDF,k is the fractional digestion rate of B, and t is the time of fermentation. Fraction C at 240 h, which is equivalent to undigested NDF (uNDF240), was determined, and fraction B was estimated as 100 – C.

      IOFC

      Income over feed costs was defined as the difference between the revenue from milk produced ($·cow−1·d−1) by milking cows and the feeding costs ($·cow−1·d−1) of milking cows (

      Ferreira, G. 2015. Income Over Feed Costs in the Dairy Enterprise. Virginia Cooperative Extension, DASC-51P. Virginia Polytech. Inst. State Univ., Blacksburg, VA.

      ). To determine revenues from milk produced, the amount of ECM (kg·d−1) produced by each cow and period was multiplied by $0.303·kg−1, which was the class III milk price for the month of December 2018 according to the Appalachian Marketing Area, class III milk price; US Federal Milk Marketing Order 5 (

      USDA. 2018. Virginia Hay Report Rushville Semimonthly Hay Auction, Harrisonburg, VA, on Wed. Jul. 18, 2018. Accessed Feb. 26, 2020. https://search.ams.usda.gov/mndms/2018/07/RH_GR31020180719.TXT.

      ). To determine feeding costs, the cost of the diet provided by the ration formulation software ($·cow−1·d−1) was divided by the predicted DMI (kg·cow−1·d−1) to obtain the cost of feed ($∙kg−1), which was then was multiplied by DMI (kg·cow−1·d−1) of each cow and period to provide the actual daily feeding cost ($·cow−1·d−1).

      Statistical Analysis

      All variables were analyzed using the MIXED procedure of SAS (SAS version 9.4, SAS Institute Inc., Cary, NC). The statistical model included the effects of square (fixed effect; df = 5), treatment (fixed effect; df = 3), square-by-treatment interaction (fixed effect; df = 15), period (random effect; df = 3), cow within square (random effect; df = 18), and the random residual error (df = 51). The treatment effect was split into orthogonal contrasts to test the main effects of hay and grain and their interaction. Significant differences between main effects were declared at P < 0.05, and significant interactions were declared at P < 0.10. When significant interactions were observed, differences among treatment means were contrasted using the pdiff option of SAS.

      RESULTS AND DISCUSSION

      According to the nutrient composition of feeds reported by

      NASEM (National Academy of Sciences, Engineering and Mathematics). 2001. Nutrient Requirements of Dairy Cattle. 7th rev. ed. Natl. Acad. Press, Washington, DC.

      , the alfalfa and grass hays used in this study to formulate the rations (Table 3) classify as mid-maturity and late-maturity hays, respectively. Considering the CP and NDF concentrations as the main parameters of forage quality, the alfalfa hay (18.0% CP and 44.6% NDF) had superior quality compared with the grass hay (8.6% CP and 77.0% NDF). However, considering the proportion of ADL in NDF as a parameter of fiber quality, the grass hay had substantially less lignification than the alfalfa hay (8.9 and 17.7%, respectively). Greater lignin concentrations in the cell walls of immature alfalfa relative to cell walls of matures grasses have been previously reported (
      • Buxton D.R.
      • Russell J.R.
      Lignin constituents and cell-wall digestibility of grass and legume stems..
      ). In agreement with the degree of lignification of the fiber, fiber digestion kinetics (Figure 1) showed that fiber digestibility at 48 h was similar for both hays and that uNDF240 was lower for grass hay than for alfalfa hay. These observations agree with previous comparisons between alfalfa and grass forages (
      • Grant R.J.
      • Weidner S.J.
      Digestion kinetics of fiber: Influence of in vitro buffer pH varied within observed physiological range..
      ;

      Van Soest, P. J. 1994. Nutritional Ecology of the Ruminant. 2nd. ed. Cornell Univ. Press, Ithaca, NY.

      ;
      • Santana O.I.
      • Olmos-Colmenero J.J.
      • Wattiaux M.A.
      Replacing alfalfa hay with triticale hay has minimal effects on lactation performance and nitrogen utilization of dairy cows in a semi-arid region of Mexico..
      ).
      Figure 1
      Figure 1In vitro residual NDF of alfalfa (solid lines and solid circles) and grass (broken line and open circles) hays. Potentially digestible NDF (PDNDF), also known as fraction B, was 53.5 and 62.4% for alfalfa hay and grass hay, respectively. Fractional digestion rate (k) of PDNDF was 7.82 and 4.32% per hour for alfalfa hay and grass hay, respectively.
      In this study, which integrates nutrition and management concepts, we hypothesized that production performance, IOFC, or both can be supported when feeding diets including grass hay instead of alfalfa hay. Analyzing production performance only, feeding diets with grass hay resulted in less ECM production (50.6 vs. 48.5 kg·d−1; P < 0.02; Table 4) and less DMI than when feeding diets with alfalfa hay (27.1 vs. 24.4 kg·d−1; P < 0.01; Table 4). It is likely, therefore, that the reduction in ECM production is related to the lower DMI observed when feeding diets containing grass hay.
      Table 4Production performance of cows consuming diets containing alfalfa or grass hays (H) and corn or wheat grains (G)
      ItemAlfalfa hayGrass haySEMP <
      Corn grainWheat grainCorn grainWheat grainHGH × G
      DMI, kg·d−127.027.125.023.81.490.010.420.30
      Milk yield, kg·d−146.348.744.345.11.650.010.060.31
      Milk fat, %4.223.564.493.950.210.010.010.61
      Milk fat yield, kg·d−11.961.751.931.740.080.640.010.85
      Milk protein, %3.023.013.033.000.090.930.680.74
      Milk protein yield, kg·d−11.401.471.301.320.050.010.130.42
      Milk lactose, %4.854.854.844.830.040.290.750.64
      Milk lactose yield, kg·d−12.282.422.092.180.110.010.060.71
      MUN, mg·dL−113.2b9.8c14.2a14.7a0.630.010.010.01
      ECM, kg·d−151.349.949.447.51.630.020.070.73
      Feed efficiency, kg of ECM·kg DMI−11.981.942.062.090.150.050.920.53
      a–cDifferent superscripts within a row differ (P < 0.05).
      When analyzing the IOFC, however, feeding diets with grass hay, instead of alfalfa hay, increased IOFC ($8.39 vs. $7.68 per cow·d−1; P < 0.03; Figure 2). The increased IOFC is explained by feeding a less expensive diet that resulted in greater milk fat concentrations (4.22 vs. 3.89% fat; P < 0.01; Table 4) and lower DMI, which compensated the decrease in revenue due to lower milk production and decreased feeding costs, respectively.
      Figure 2
      Figure 2Income over feed costs of milking cows consuming diets containing alfalfa or grass hays and corn or wheat grains. Prices of alfalfa and grass hays were $418 and $154·t−1, respectively. Milk price was determined considering fat and skim milk prices according to Federal Milk Order 5 (

      USDA. 2018. Virginia Hay Report Rushville Semimonthly Hay Auction, Harrisonburg, VA, on Wed. Jul. 18, 2018. Accessed Feb. 26, 2020. https://search.ams.usda.gov/mndms/2018/07/RH_GR31020180719.TXT.

      ). Only the main effect of hay was significant (P < 0.03; SEM = 0.65).
      Milk fat concentrations decreased when wheat grain replaced corn grain in diets fed to dairy cows (
      • Gozho G.N.
      • Mutsvangwa T.
      Influence of carbohydrate source on ruminal fermentation characteristics, performance, and microbial protein synthesis in dairy cows..
      ;
      • Moate P.J.
      • Jacobs J.L.
      • Hannah M.C.
      • Morris G.L.
      • Beauchemin K.A.
      • Alvarez Hess P.S.
      • Eckard R.J.
      • Liu Z.
      • Rochfort S.
      • Wales W.J.
      • Williams S.R.O.
      Adaptation responses in milk fat yield and methane emissions of dairy cows when wheat was included in their diet for 16 weeks..
      ). In agreement, in this study, a decrease in milk fat concentration existed when feeding wheat grain instead of corn grain. Although ECM only tended to decrease when feeding wheat grain, no interaction between hay type and grain type existed for ECM. This lack of interaction in ECM translated into a lack of interaction between hay type and grain type on IOFC.
      The difference in DMI is likely related to the difference in dietary concentrations of forage NDF, which were 17.3 and 23.8% of DM for diets containing alfalfa or grass hay, respectively. Such difference is attributed to the different inclusion rates of hay in the diets (14.1 and 19.2% for alfalfa and grass hays, respectively) and to the different NDF concentrations in the hays (44.6 and 75.0% for alfalfa and grass hays, respectively). Greater inclusions of a more fibrous hay, such as the grass hay in this study, likely increased ruminal retention times of forage particles that would slow down rumen turnover and decrease DMI.
      While a difference in DMI in our study should be anticipated based on the different hay inclusion rates and the different NDF concentrations of the hays, nutritionists frequently claim that cows require nutrients and not ingredients. Therefore, it should be noted that both diets met the minimum requirements for NDF and forage NDF concentrations (

      NASEM (National Academy of Sciences, Engineering and Mathematics). 2001. Nutrient Requirements of Dairy Cattle. 7th rev. ed. Natl. Acad. Press, Washington, DC.

      ) and that both diets complied with the least-cost formulation constraints. In line with this concept,
      • Eastridge M.L.
      • Bucci P.B.
      • Ribeiro C.
      Feeding equivalent concentrations of forage neutral detergent fiber from alfalfa hay, grass hay, wheat straw, and whole cottonseed in corn silage based diets to lactating cows..
      fed lactating cows diets containing either alfalfa hay (19.0% CP and 38.8% NDF) or grass hay (12.4% CP and 58.3% NDF) but that were similar in dietary CP, NDF, and forage NDF concentrations and reported no changes in DMI or milk yields.
      Results from this and other studies (
      • Grant R.J.
      • Weidner S.J.
      Digestion kinetics of fiber: Influence of in vitro buffer pH varied within observed physiological range..
      ;
      • Santana O.I.
      • Olmos-Colmenero J.J.
      • Wattiaux M.A.
      Replacing alfalfa hay with triticale hay has minimal effects on lactation performance and nitrogen utilization of dairy cows in a semi-arid region of Mexico..
      ) may help challenge the misconception that grass hays have poor nutritional quality and are not suitable for feeding high-producing dairy cattle. If evaluated based only on its low energy or protein concentrations, then grass hay could be considered a poor-quality forage. For example, when considering CP (
      • Grant R.J.
      Influence of corn and sorghum starch on the in vitro kinetics of forage fiber digestion..
      ;
      • Eastridge M.L.
      • Bucci P.B.
      • Ribeiro C.
      Feeding equivalent concentrations of forage neutral detergent fiber from alfalfa hay, grass hay, wheat straw, and whole cottonseed in corn silage based diets to lactating cows..
      ) or cell content (
      • Smith L.W.
      • Goering H.K.
      • Waldo D.R.
      • Gordon C.H.
      In vitro digestion rate of forage cell wall components..
      ;
      • Buxton D.R.
      • Russell J.R.
      Lignin constituents and cell-wall digestibility of grass and legume stems..
      ) concentrations, alfalfa hay would likely be considered of better quality than grass hay. However, grass-based forages may show greater ruminal NDF digestibility than alfalfa-based forages (
      • Mertens D.R.
      • Loften J.R.
      The effect of starch on forage fiber digestion kinetics in vitro..
      ;
      • Grant R.J.
      Influence of corn and sorghum starch on the in vitro kinetics of forage fiber digestion..
      ; Voelker-Linton and Allen, 2008;
      • Kammes K.L.
      • Allen M.S.
      Nutrient demand interacts with grass particle length to affect digestion responses and chewing activity in dairy cows..
      ) depending on forage maturity at harvesting (
      • Buxton D.R.
      • Russell J.R.
      Lignin constituents and cell-wall digestibility of grass and legume stems..
      ). Therefore, grass hay could still be strategically included in the diet of high-producing dairy cows to ensure rumen health and optimize nutrient utilization. Even more, in many modern dairy farming systems, hay is unlikely to be the only or major energy and protein source in the diet and rather is a component of a complex ration. In this context, grass hay may be a very good source of potentially digestible NDF (

      Van Soest, P. J. 1994. Nutritional Ecology of the Ruminant. 2nd. ed. Cornell Univ. Press, Ithaca, NY.

      ). To highlight this, multiple studies have shown that the concentration of undegradable NDF (NDF basis) is lower for grasses than for alfalfa (
      • Grant R.J.
      • Weidner S.J.
      Digestion kinetics of fiber: Influence of in vitro buffer pH varied within observed physiological range..
      ;

      Van Soest, P. J. 1994. Nutritional Ecology of the Ruminant. 2nd. ed. Cornell Univ. Press, Ithaca, NY.

      ;
      • Santana O.I.
      • Olmos-Colmenero J.J.
      • Wattiaux M.A.
      Replacing alfalfa hay with triticale hay has minimal effects on lactation performance and nitrogen utilization of dairy cows in a semi-arid region of Mexico..
      ). Similarly, in this study grass hay had a lower undegradable NDF concentration (NDF basis) than alfalfa hay (37.7 and 46.4%, respectively; Figure 1). In regard to effective ruminal degradability (ERD), whereas
      • Santana O.I.
      • Olmos-Colmenero J.J.
      • Wattiaux M.A.
      Replacing alfalfa hay with triticale hay has minimal effects on lactation performance and nitrogen utilization of dairy cows in a semi-arid region of Mexico..
      reported similar ERD for grass and alfalfa hays,
      • Grant R.J.
      • Weidner S.J.
      Digestion kinetics of fiber: Influence of in vitro buffer pH varied within observed physiological range..
      reported greater ERD for grass hay than for alfalfa hay. In this study, assuming a passage rate for potentially digestible NDF of 2%·h−1 (
      • Kammes K.L.
      • Allen M.S.
      Nutrient demand interacts with grass particle length to affect digestion responses and chewing activity in dairy cows..
      ), grass hay and alfalfa hay had similar ERD (42.7% NDF). Therefore, these observations clearly show that, from a fiber digestion kinetics perspective, there are no reasons to imply that grass hay has poorer quality than alfalfa hay.
      An interaction between grain and hay existed for NDF digestibility (Table 5). To explain this interaction, we considered the diet with corn grain plus alfalfa hay as a reference diet to explain individual contrasts. The diet with corn grain plus grass hay resulted in a reduced NDF digestibility, relative to the diet with corn grain plus alfalfa hay. This reduced NDF digestibility may be attributed to differences in the proportion of forage NDF in the diet rather than to differences in the NDF digestibility of the hays per se. Specifically, in the diet with corn grain plus grass hay, forages provided 79.6% of the dietary NDF, whereas in the diet with corn grain plus alfalfa hay, forages provided 60.6% of the dietary NDF (Table 1). Although speculative, a greater proportion of lignified NDF from forage sources could explain the reduced NDF digestibility observed for the diet with corn grain plus grass hay. The diet with wheat grain plus grass hay had an NDF digestibility coefficient similar to that of the diet with corn grain plus alfalfa hay (Table 5). In this case, even though forages provided much more NDF (74.8 vs. 60.6%), the faster fermentability of wheat grain may have provided more energy to the rumen microbes to ferment dietary or forage fiber, which may have reduced the flux of fiber to the intestine. Finally, the diet with wheat grain plus alfalfa hay resulted in a reduced NDF digestibility relative to the diet with corn grain plus alfalfa hay. This reduction in NDF digestibility could be attributed to the compounding of a lower forage NDF concentration and a lower ruminal pH (
      • Grant R.J.
      • Weidner S.J.
      Digestion kinetics of fiber: Influence of in vitro buffer pH varied within observed physiological range..
      ) possibly caused by the rapid fermentability of wheat grain (
      • Herrera-Saldana R.E.
      • Huber J.T.
      • Poore M.H.
      Dry matter, crude protein, and starch degradability of five cereal grains..
      ). The very high digestibility coefficients observed in this study should be noted (Table 5). In support of these data, we used the same methodology as described previously (
      • Yang Y.
      • Ferreira G.
      • Teets C.L.
      • Corl B.A.
      • Thomason W.E.
      • Griffey C.A.
      Effects of feeding hull-less barley on production performance, milk fatty acid composition, and nutrient digestibility of lactating dairy cows..
      ,
      • Yang Y.
      • Ferreira G.
      • Teets C.L.
      • Corl B.A.
      • Thomason W.E.
      • Griffey C.E.
      Effects of feeding hulled and hull-less barley with low- and high-forage diets on lactation performance, nutrient digestibility, and milk fatty acid composition of lactating dairy cows..
      ,
      • Yang Y.
      • Ferreira G.
      • Corl B.A.
      • Campbell B.T.
      Production performance, nutrient digestibility, and milk fatty acid profile of lactating dairy cows fed corn silage- or sorghum silage-based diets with and without xylanase supplementation..
      ). Also, we screened the raw data, but found no outliers or suspicious data. Therefore, even though these digestibility coefficients are unusually high, we did not find any analytical reasons to discard them.
      Table 5Apparent total-tract digestibility of cows consuming diets containing alfalfa or grass hays (H) and corn or wheat grains (G)
      ItemAlfalfa hayGrass haySEMP <
      Corn grainWheat grainCorn grainWheat grainHGH × G
      DM, %79.8a78.8a73.9b80.1a1.150.010.010.01
      CP, %76.2b78.1a68.5c76.9ab0.850.010.010.01
      NDF, %64.0a59.0b53.8c64.8a2.900.070.020.01
      Starch, %99.1b99.4a98.6c99.6a0.180.060.010.01
      a–cDifferent superscripts within a row differ (P < 0.05).
      From a management perspective, feeding grass hay increased IOFC in this study. In addition to the lower diet cost (Table 1) and the lower DMI (Table 4), the greater IOFC of cows consuming diets with grass hay is explained by the increased concentration of fat in milk and, hence, increased fat yield (Table 4). Relative to feeding diets with alfalfa hay, a slight increase of milk fat concentration when feeding grass-based diets has been reported in some (
      • Santana O.I.
      • Olmos-Colmenero J.J.
      • Wattiaux M.A.
      Replacing alfalfa hay with triticale hay has minimal effects on lactation performance and nitrogen utilization of dairy cows in a semi-arid region of Mexico..
      ) but not all studies (
      • Broderick G.A.
      • Koegel R.G.
      • Walgenbach R.P.
      • Kraus T.J.
      Ryegrass or alfalfa silage as the dietary forage for lactating dairy cows..
      ;
      • Zhu W.
      • Fu Y.
      • Wang B.
      • Wang C.
      • Ye J.A.
      • Wu Y.M.
      • Liu J.X.
      Effects of dietary forage sources on rumen microbial protein synthesis and milk performance in early lactating dairy cows..
      ;
      • Bender R.W.
      • Lopes F.
      • Cook D.E.
      • Combs D.K.
      Effects of partial replacement of corn and alfalfa silage with tall fescue hay on total-tract digestibility and lactation performance in dairy cows..
      ). Therefore, greater milk fat concentrations when feeding grass hay, compared with alfalfa hay, in diets might be variable, as confounding effects between forage type and dietary forage NDF have been reported (
      • Zebeli Q.
      • Tafaj M.
      • Steingass H.
      • Metzler B.
      • Drochner W.
      Effects of physically effective fiber on digestive processes and milk fat content in early lactating dairy cows fed total mixed rations..
      ). However, regardless of an increase of IOFC, results from this study highlight that the inclusion of alfalfa hay is not necessarily critical to support production performance and IOFC, especially in geographical regions where these feed resources are scarce and, hence, costly.

      APPLICATIONS

      This study evaluated the inclusion of alfalfa versus grass hays in a TMR for high-producing dairy cows using a least-cost formulation approach to find alternative feeding practices that maximize IOFC. Despite the differences in quality between the 2 hays, results from this study showed that IOFC can be supported with less-expensive locally grown hay, such as mixed-grass hay, when using a least-cost formulation approach. One major reason for this is that, in dairy farming systems, hays are a component of a diet and not a major ingredient, as in several cow-calf beef farming systems. Another reason is that cows require nutrients and not ingredients. Therefore, when physically effective fiber is required, grass hay can be a suitable ingredient in properly formulated rations for high-producing dairy cows.

      ACKNOWLEDGMENTS

      We are extremely thankful to Virginia Tech undergraduate students Claudia Bollinger, Kirby Lingenfelser, and Elizabeth Pittman for their assistance feeding the cows and collecting samples. This project was funded mostly by the Virginia Agricultural Council (Project No. 718—Effects of feeding wheat grain on production performance and nutrient digestibility of lactating dairy cows consuming diets containing high- and low-quality hay) and partially by USDA-NIFA Multistate Project VA-136291 (NC-2042 Management Systems to Improve the Economic and Environmental Sustainability of Dairy Enterprises).

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