ABSTRACT
Objective
The objectives were to quantify collagen content in nonlactating uninfected and Staphylococcus aureus–infected mammary glands and determine the relative abundance of collagen in different
gland regions.
Materials and Methods
Mammary tissues that were previously collected from a mastitis-challenge trial were
examined. Mammary tissues were collected along a vertical midline through each gland
and included parenchymal tissues that were proximal to the gland cistern (cisternal
parenchyma) and tissues near the abdominal wall (deep parenchyma) from each saline-infused
(n = 18) and Staph. aureus–infused (n = 18) gland. Collagen was detected via picro-sirus red staining and visualized
using polarized light for image acquisition and subsequent analysis.
Results and Discussion
Most interlobular collagen was associated with larger ducts and stromal tissues. Some
collagen was intralobular and was mostly associated with these larger ducts but was
also present in epithelial basement membranes. Staphylococcus aureus–infused glands contained more tissue area occupied by collagen than saline-infused
glands (19.78 vs. 17.26 ± 1.19%). Additionally, parenchyma near the gland cistern
contained more collagen than deep parenchyma (19.95 vs. 17.09 ± 1.19%). Results indicate
that mastitis elicits the deposition of collagen in affected glands and that collagen
abundance is affected by gland region.
Implications and Applications
Nonlactating mammary glands affected by Staph. aureus mastitis may have a greater amount of collagen, believed to be resulting scar tissue.
We speculate that it may have long-term, and possibly irreversible, effects on glandular
structure and, consequently, lifetime productivity.
Key words
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Article info
Publication history
Accepted:
July 20,
2020
Received:
March 2,
2020
Footnotes
The authors declare no conflicts of interest.
Identification
Copyright
© 2020 American Registry of Professional Animal Scientists. Published by Elsevier Inc. All rights reserved.
