ABSTRACT
Objective
We evaluated the effects of adding live Saccharomyces cerevisiae (SC) and Aspergillus-based extract (ABE) on rumen fermentation, plasma polyamine concentrations, and in
vitro fiber digestibility in beef steers fed mixed red clover–orchardgrass hay.
Materials and Methods
Eight rumen-cannulated Angus crossbred beef steers were assigned to 4 treatments arranged
in a replicated 4 × 4 Latin square design with four 21-d periods. Dietary treatments
were (1) control (CON; basal diet without additive), (2) SC (CON + 15 g/d of live
SC), (3) ABE (5 g of ABE), and (4) SC+ABE (15 g/d of live SC + 5 g of ABE). On d 21,
blood samples were collected before morning feeding for plasma metabolome analysis
(including polyamines such as spermines, spermidine, and putrescine). Rumen fluid
samples were collected 3, 6, and 9 h after feeding for analysis of VFA, ammonia-N
concentrations, and in vitro DM and NDF digestibility.
Results and Discussion
Compared with CON, neither SC nor ABE had effects (P > 0.10) on rumen fermentation and in vitro digestibility. In contrast, SC+ABE increased
(P ≤ 0.05) the ruminal concentrations of acetate, butyrate, and total VFA and in vitro
DM and NDF digestibilities. Both SC and SC+ABE increased (P < 0.05) plasma concentrations of spermidine, spermine, and kynurenine.
Implications and Applications
Supplementation of SC with or without ABE to forage-fed beef steers increased plasma
concentrations of polyamines and kynurenine, a possible indication of improved immune
function; however, combining SC and ABE, but not SC alone, improved the rumen fermentation
and in vitro fiber digestibility.
Key words
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Article info
Publication history
Accepted:
November 13,
2020
Received:
April 29,
2020
Footnotes
The authors declare no conflicts of interest.
Identification
Copyright
© 2021 American Registry of Professional Animal Scientists. Published by Elsevier Inc. All rights reserved.
