ABSTRACT
Objective
Our objective was to determine the effect of dietary Zn supplementation on microbial
fermentation.
Materials and Methods
Five dual-flow continuous culture fermentors were offered 15 g of DM/d of a corn silage–based
diet that supplied 64 mg of Zn/kg of diet DM. The source of supplemental Zn (30 and
120 mg/kg) was either analytical grade zinc oxide (ZnO) or a greater-purity potentiated
zinc oxide (HiZox; Animine, Annecy, France). A total of 5 diets were fermented: (1)
control (no supplemental Zn); (2) 30 mg/kg supplemental Zn from ZnO; (3) 120 mg/kg
supplemental Zn from ZnO; (4) 30 mg/kg supplemental Zn from HiZox, and (5) 120 mg/kg
supplemental Zn from HiZox. Diets were replicated (n = 3), and each replication lasted
8 d with 4 d for adjustment to diets followed by 4 d of data collection.
Results and Discussion
Rumen soluble Zn (as-is basis) increased with supplemental Zn, and the increase tended
to be greater with HiZox compared with ZnO. Total VFA concentrations were not affected
by treatment. Molar percentage of acetate increased and propionate decreased with
HiZox compared with ZnO; the effects were primarily due to the 120 mg/kg concentration.
Zinc oxide decreased branched-chain fatty acids, isobutyrate and isovalerate, when
compared with HiZox. Supplemental Zn increased culture pH before and after feeding
compared with control; the increase was greatest with 30 mg/kg HiZox. Methane was
similar between control and HiZox but significantly reduced with ZnO. Zinc supplementation
reduced ammonia-N concentration when compared with control; the decrease was due primarily
to ZnO, which resulted in much lower ammonia-N compared with HiZox.
Implications and Applications
Both supplemental Zn sources increased rumen-soluble Zn (as-is basis), but their effect
on fermentation was not similar. The divalent Zn2+ is known to combine with Cl− to form various Zn-Cl complexes (ZnCl+, ZnCl2, ZnCl3−), which may exert differing toxicities and explain the disparate effect on microbial
fermentation.
Key words
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Article info
Publication history
Accepted:
December 7,
2020
Received:
September 30,
2020
Footnotes
Two of the authors are employed by Animine, which provided funding for this research. The other authors declare no conflicts of interest.
Identification
Copyright
© 2021 American Registry of Professional Animal Scientists. Published by Elsevier Inc. All rights reserved.
