Effects of supplemental zinc on ruminal fermentation in continuous cultures*



      Our objective was to determine the effect of dietary Zn supplementation on microbial fermentation.

      Materials and Methods

      Five dual-flow continuous culture fermentors were offered 15 g of DM/d of a corn silage–based diet that supplied 64 mg of Zn/kg of diet DM. The source of supplemental Zn (30 and 120 mg/kg) was either analytical grade zinc oxide (ZnO) or a greater-purity potentiated zinc oxide (HiZox; Animine, Annecy, France). A total of 5 diets were fermented: (1) control (no supplemental Zn); (2) 30 mg/kg supplemental Zn from ZnO; (3) 120 mg/kg supplemental Zn from ZnO; (4) 30 mg/kg supplemental Zn from HiZox, and (5) 120 mg/kg supplemental Zn from HiZox. Diets were replicated (n = 3), and each replication lasted 8 d with 4 d for adjustment to diets followed by 4 d of data collection.

      Results and Discussion

      Rumen soluble Zn (as-is basis) increased with supplemental Zn, and the increase tended to be greater with HiZox compared with ZnO. Total VFA concentrations were not affected by treatment. Molar percentage of acetate increased and propionate decreased with HiZox compared with ZnO; the effects were primarily due to the 120 mg/kg concentration. Zinc oxide decreased branched-chain fatty acids, isobutyrate and isovalerate, when compared with HiZox. Supplemental Zn increased culture pH before and after feeding compared with control; the increase was greatest with 30 mg/kg HiZox. Methane was similar between control and HiZox but significantly reduced with ZnO. Zinc supplementation reduced ammonia-N concentration when compared with control; the decrease was due primarily to ZnO, which resulted in much lower ammonia-N compared with HiZox.

      Implications and Applications

      Both supplemental Zn sources increased rumen-soluble Zn (as-is basis), but their effect on fermentation was not similar. The divalent Zn2+ is known to combine with Cl to form various Zn-Cl complexes (ZnCl+, ZnCl2, ZnCl3), which may exert differing toxicities and explain the disparate effect on microbial fermentation.

      Key words

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        • Allison M.J.
        Production of branched-chain volatile fatty acids by certain anaerobic bacteria..
        Appl. Environ. Microbiol. 1978; 35: 872-877
        • Arelovich H.M.
        • Owens F.N.
        • Horn G.W.
        • Vizcarra J.A.
        Effects of supplemental zinc and manganese on ruminal fermentation, forage intake, and digestion by cattle fed prairie hay and urea..
        J. Anim. Sci. 2000; 78: 2972-2979
        • Babich H.
        • Stotzky G.
        Toxicity of zinc to fungi, bacteria, and coliphages: Influence of chloride ions..
        Appl. Environ. Microbiol. 1978; 36: 906-914
        • Beecher G.R.
        • Whitten B.K.
        Ammonia determination: Reagent modification and interfering compounds..
        Anal. Biochem. 1970; 36: 243-246
        • Blümmel M.
        • Makkar H.P.S.
        • Becker K.
        In vitro gas production: A technique revisited..
        J. Anim. Physiol. 1997; 77: 24-34
        • Caldera E.
        • Weigel B.
        • Kucharczyk V.N.
        • Sellins K.S.
        • Archibeque S.L.
        • Wagner J.J.
        • Han H.
        • Spears J.W.
        • Engle T.E.
        Trace mineral source influences ruminal distribution of copper and zinc and their binding strength to ruminal digesta..
        J. Anim. Sci. 2019; 97: 1852-1864
        • Callander I.J.
        • Barford J.P.
        Precipitation, chelation, and the availability of metals as nutrients in anaerobic digestion. I. Methodology..
        Biotechnol. Bioeng. 1983; 25: 1947-1957
      1. Campbell, C. R., and C. O. Plank. 1992. Sample preparation. Pages 1–12 in Plant Analysis Reference Procedures for the Southern Region of the United States. Southern Cooperative Series Bulletin 368. Georgia Coop. Ext. Serv., Athens, GA.

        • Cao J.
        • Henry P.R.
        • Guo R.
        • Holwerda R.A.
        • Toth J.P.
        • Littell R.C.
        • Miles R.D.
        • Ammerman C.B.
        Chemical characteristics and relative bioavailability of supplemental organic zinc sources for poultry and ruminants..
        J. Anim. Sci. 2000; 78: 2039-2054
      2. Cardoso, D., Y. Chevalier, A. Narcy, A. Romeo, and S. Durosoy. 2017. The effect of physicochemical properties of feed grade zinc oxide sources in dissolution kinetics. Presented at the 16th Int. Symp. Trace Elements Man Anim., Saint Petersburg, Russia.

        • Choudhury R.
        • Srivastava S.
        Zinc resistance mechanisms in bacteria..
        Curr. Sci. 2001; 81: 768-775
        • Czerkawski J.W.
        • Breckenridge G.
        Design and development of a long-term rumen simulation technique (Rusitec)..
        Br. J. Nutr. 1977; 38: 371-384
      3. Durand, M., and R. Kawashima. 1980. Influence of minerals on rumen microbial digestion. Pages 375–408 in Digestive Physiology and Metabolism in Ruminants. Y. Ruckebusch and P. Thivend, ed. AVI Publ. Co., Westport, CT. 10.1007/978-94-011-8067-2_18.

        • Eryavuz A.
        • Dehority B.A.
        Effects of supplemental Zn concentration on cellulose digestion and cellulolytic and total bacterial numbers in vitro..
        Anim. Feed Sci. Technol. 2009; 151: 175-183
        • Eun J.-S.
        • Fellner V.
        • Burns J.C.
        • Gumpertz M.L.
        Fermentation of eastern gamagrass (Tripsacum dactyloides [L.] L.) by mixed cultures of ruminal microorganisms with or without supplemental corn..
        J. Anim. Sci. 2004; 82 (a): 170-178
        • Eun J.-S.
        • Fellner V.
        • Gumpertz M.L.
        Methane production by mixed ruminal cultures incubated in dual-flow fermentors..
        J. Dairy Sci. 2004; 87 (b): 112-121
        • Hubbert F.
        • Cheng E.
        • Burroughs W.
        Mineral requirements of rumen microorganisms for cellulose digestion in vitro..
        J. Anim. Sci. 1958; 17: 559-568
        • Kennedy D.W.
        • Craig W.M.
        • Southern L.L.
        Ruminal distribution of zinc in steers fed a polysaccharide-zinc complex or zinc oxide..
        J. Anim. Sci. 1993; 71: 1281-1287
        • Latham M.J.
        • Sutton J.D.
        • Sharpe M.E.
        Fermentation and microorganisms in the rumen and the content of milk of cows given low roughage rations..
        J. Dairy Sci. 1974; 57: 803-810
        • Little O.
        • Cheng E.
        • Burroughs W.
        Effects of chelating agents on cellulose digestion in vitro by rumen microorganisms..
        J. Anim. Sci. 1958; 17 (Abstr.): 1190
        • Liu Q.
        • Wang C.
        • Pei C.X.
        • Li H.Y.
        • Wang Y.X.
        • Zhang S.L.
        • Zhang Y.L.
        • He J.P.
        • Wang H.
        • Yang W.Z.
        • Bai Y.S.
        • Shi Z.G.
        • Liu X.N.
        Effects of isovalerate supplementation on microbial status and rumen enzyme profile in steers fed on corn stover based diet..
        Livest. Sci. 2014; 161: 60-68
        • Martinez A.
        • Church D.C.
        Effect of various mineral elements on in vitro rumen cellulose digestion..
        J. Anim. Sci. 1970; 31: 982-990
      4. NASEM (National Academies of Sciences, Engineering, and Medicine). 2001. Nutrient Requirements of Dairy Cattle. 7th rev. ed. ed. Natl. Acad. Sci., Washington, DC. 10.17226/9825.

        • Slyter L.L.
        • Bryant M.P.
        • Wolin M.J.
        Effect of pH on population and fermentation in a continuously cultured rumen ecosystem..
        Appl. Microbiol. 1966; 14: 573-578
        • Spears J.W.
        • Kegley E.B.
        Effect of zinc source (zinc oxide vs zinc proteinate) and level on performance, carcass characteristics, and immune response of growing and finishing steers..
        J. Anim. Sci. 2002; 80: 2747-2752
        • Spears J.W.
        • Schlegel P.
        • Seal M.C.
        • Lloyd K.E.
        Bioavailability of zinc from zinc sulfate and different organic zinc sources and their effects on ruminal volatile fatty acid proportions..
        Livest. Prod. Sci. 2004; 90: 211-217
      5. US EPA (United States Environmental Protection Agency). 2001. Method 200.7. Trace Elements in Water, Solids, and Biosolids by Inductively Coupled Plasma–Atomic Spectrometry. Revision 4.4. EPA-821-R-01-010. US Environ. Prot. Agency Off. Res. Dev., Cincinnati, OH.

        • Wang R.L.
        • Liang J.G.
        • Lu L.
        • Zhang Y.
        • Li S.F.
        • Luo X.G.
        Effect of zinc source on performance, zinc status, immune response, and rumen fermentation of lactating cows..
        Biol. Trace Elem. Res. 2013; 152: 16-24
      6. Woods, W. 1965. Study of the Efficiency of Various Sources of Trace Elements for Ruminant Animals. Dept. Anim. Sci. Rep. No. 3 on Project No. ZC87. Univ. Nebraska, Lincoln.

        • Zerebcov P.I.
        • Nabiev N.H.
        • Vlijanie N.H.
        Effect of different amounts of Zn on N and carbohydrate metabolism in the rumen of cattle..
        Nutr. Abstr. Rev. 1971; 41: 130