ABSTRACT
Objective
The primary aim of this work was to compare the plasma Met response to supplementation
with 3 rumen-protected Met products. Secondary aims were to evaluate how time of sampling
affected results and how pooling of samples may have affected results.
Materials and Methods
Ten multiparous Holstein cows (280 ± 73 DIM) were used in a replicated 3 × 3 Latin
square design with 7-d experimental periods. Cows were fed every 8 h, and treatments
consisted of supplementation of 12 g/d (4 g/feeding) of 1 of 3 rumen-protected Met
(RPM) products. The products evaluated were the newly developed RPM-K and 2 existing
products, RPM-S and RPM-M, with known differences in bioavailability. During d 5 to
7 of each period, blood samples were collected at 2, 4, 6, and 8 h after the morning
feeding for plasma free AA analysis. Plasma Met data were analyzed using the full
data set as well as mean values from individual cows for each day or each period.
Results and Discussion
Plasma Met was not different between RPM-S and RPM-K (32.7 vs. 33.0 μM, respectively; P = 0.79), and both were greater than RPM-M (30.1 μM; P ≤ 0.001). Plasma Met was affected by time of sampling (P = 0.001), due to reduced plasma Met at 4 h (30.2 μM) than at 2, 6, and 8 h (31.9–33.0 μM). Using the daily and period mean values of plasma Met, differences observed in the
full model were maintained when daily means were evaluated, but period means resulted
in only a tendency for a treatment effect.
Implications and Applications
Bioavailability of RPM-K was similar to RPM-S and greater than RPM-M. Pooling samples
by day within cow would have likely yielded similar results.
Key words
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LITERATURE CITED
- Postprandial portal fluxes of essential amino acids, volatile fatty acids, and urea-nitrogen in growing pigs fed a high-fiber diet supplemented with a multi-enzyme cocktail..https://doi.org/10.2527/jas.2015-007727898914J. Anim. Sci. 2016; 94: 3771-3785
AOAC International. 2000. Official Methods of Analysis of AOAC International. 17th ed. AOAC Int.
- Measuring resistance to ruminal degradation and bioavailability of ruminally protected methionine..https://doi.org/10.1016/S0377-8401(00)00113-9Anim. Feed Sci. Technol. 2000; 84: 23-32
- Comparison of the in situ and in vivo intestinal disappearance of ruminally protected methionine..https://doi.org/10.3168/jds.S0022-0302(00)75086-711003238J. Dairy Sci. 2000; 83: 2049-2056
- Rumen-protected methionine fed to dairy cows: Bioavailability and effects on plasma amino acid pattern and plasma metabolite and insulin concentrations..https://doi.org/10.3168/jds.S0022-0302(99)75435-410509258J. Dairy Sci. 1999; 82: 1991-1998
- Methionine availability in plasma of dairy cows supplemented with methionine hydroxy analog isopropyl ester..https://doi.org/10.3168/jds.S0022-0302(05)73049-616162538J. Dairy Sci. 2005; 88: 3640-3649
- Determination of starch, including maltooligosaccharides, in animal feeds: comparison of methods and a method recommended for AOAC collaborative study..https://doi.org/10.1093/jaoac/92.1.4219382561J. AOAC Int. 2009; 92: 42-49
- Ruminal degradability, intestinal disappearance, and plasma methionine response of rumen-protected methionine in dairy cows..https://doi.org/10.3168/jds.S0022-0302(01)70181-611417708J. Dairy Sci. 2001; 84: 1480-1487
- Amino acid determination in biological fluids by automated ion-exchange chromatography: Performance of Hitachi L-8500A..https://doi.org/10.1093/clinchem/43.8.14219267323Clin. Chem. 1997; 43: 1421-1428
- Degradation of methionine by rumen contents in vitro and efficiency of its protection..https://doi.org/10.1016/S0377-8401(97)00008-4Anim. Feed Sci. Technol. 1997; 67: 339-347
NASEM (National Academies of Sciences, Engineering, and Medicine). 2001. Nutrient Requirements of Dairy Cattle. 7th rev. ed. Natl. Acad. Press.
- Effects of providing two forms of supplemental methionine to periparturient Holstein dairy cows on feed intake and lactational performance..https://doi.org/10.3168/jds.2009-225919762834J. Dairy Sci. 2009; 92: 5154-5166
- Evaluation of a ruminally protected methionine product for lactating dairy cows..https://doi.org/10.3168/jds.S0022-0302(96)76408-18744228J. Dairy Sci. 1996; 79: 631-638
- Effectiveness of rumen-protected methionine for delivering methionine postruminally in dairy cows..https://doi.org/10.3168/jds.S0022-0302(84)81337-5J. Dairy Sci. 1984; 67: 545-552
- Bioavailability of rumen-protected methionine, lysine and histidine assessed by fecal amino acid excretion..https://doi.org/10.1016/j.anifeedsci.2020.114595Anim. Feed Sci. Technol. 2020; 268
- Portal net appearance of amino acids in growing pigs fed a barley-based diet with inclusion of three different forage meals..https://doi.org/10.1017/S000711450000179311103219Br. J. Nutr. 2000; 84: 483-494
- Development of a method for measuring lysine and methionine bioavailability in rumen-protected products for cattle..https://doi.org/10.22358/jafs/67723/2003J. Anim. Feed Sci. 2003; 12: 465-474
Schwab, C. G. 1995. Protected proteins and amino acids for ruminants. Pages 115–141 in Biotechnology in Animal Feeds and Animal Feeding. R. J. W. a. A. Chesson, ed. VCH.
- A 100-Year Review: Protein and amino acid nutrition in dairy cows..https://doi.org/10.3168/jds.2017-1332029153157J. Dairy Sci. 2017; 100: 10094-10112
- Bioavailability of three ruminally protected methionine sources in cattle..https://doi.org/10.1016/j.anifeedsci.2003.11.001Anim. Feed Sci. Technol. 2004; 113: 17-25
- Effect of feeding rumen-protected methionine on productive and reproductive performance of dairy cows..https://doi.org/10.1371/journal.pone.018911729261700PLoS One. 2017; 12
- Effects of feeding rumen-protected methionine pre- and postpartum in multiparous Holstein cows: Lactation performance and plasma amino acid concentrations..https://doi.org/10.3168/jds.2020-1902133865588J. Dairy Sci. 2021; 104: 7583-7603
- The Cornell Net Carbohydrate and Protein System: Updates to the model and evaluation of version 6.5..https://doi.org/10.3168/jds.2015-937826142847J. Dairy Sci. 2015; 98: 6361-6380
- Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition..https://doi.org/10.3168/jds.S0022-0302(91)78551-21660498J. Dairy Sci. 1991; 74: 3583-3597
- The plasma free amino acid dose-response technique: A proposed methodology for determining lysine relative bioavailability of rumen-protected lysine supplements..https://doi.org/10.3168/jds.2017-1269528964520J. Dairy Sci. 2017; 100: 9585-9601
- Plasma flow and net nutrient flux across gut and liver of cattle fed twice daily..https://doi.org/10.2527/1996.74102450x8904714J. Anim. Sci. 1996; 74: 2450-2461
- Meta-analysis of lactation performance in dairy cows receiving supplemental dietary methionine sources or postruminal infusion of methionine..https://doi.org/10.3168/jds.2014-822025242429J. Dairy Sci. 2014; 97: 7085-7101
Article info
Publication history
Accepted:
December 22,
2021
Received:
August 26,
2021
Footnotes
Three of the authors work for Kemin, which provided funding for this research. The other authors have not declared any conflicts of interest.
Identification
Copyright
© 2022 American Registry of Professional Animal Scientists. Published by Elsevier Inc. All rights reserved.
