ABSTRACT
Objective
The objectives of this study were to elucidate the efficiency of oocyte recovery through
different techniques, grading of oocytes, and optimization of the caprine in vitro
embryo production system.
Materials and Methods
Ovaries were collected from the local slaughterhouse and classified based on the presence
of a corpus luteum (CL). A total of 442 cumulus-oocyte complexes (COC) were retrieved
from 178 ovaries aseptically through aspiration, slicing, and slashing techniques.
Collected COC were categorized according to the compactness of cumulus cells around
the oocyte and the homogeneous cytoplasm. In vitro maturation of graded COC was done
for 27 h. In vitro fertilization (IVF) of the matured oocytes was performed using
frozen semen. At 16 h of IVF, presumptive zygotes were in vitro cultured for 7 d.
Results and Discussion
It was found that 31% of ovaries had a CL and 69% did not have a CL. The number of
oocytes recovered per ovary (2.89 ± 0.22) and the percentage of excellent oocytes,
grade A (29.84 ± 4.00%) and B (44.80 ± 2.32%), were significantly greater from the
slashing technique than from the aspiration (2.16 ± 0.30 and 11.80 ± 0.61%, 30.80 ± 3.56%, respectively) and slicing technique (2.40 ± 0.15 and 22.62 ± 7.81%, 16.10 ± 1.29%, respectively). Results revealed a significantly
greater (P < 0.05) rate of grade A (61.14 ± 5.83%, 36.04 ± 0.32%, and 22.51 ± 3.07%) and grade
B oocytes (23.31 ± 1.61%, 28.90 ± 7.47%, and 28.29 ± 5.04%) reached the metaphase
II stage recovered from 3 methods (slashing, slicing and aspiration, respectively).
The cleavage and blastocyst rates of presumptive zygotes were found to be 74.15 ±
6.80% and 37.50%, respectively.
Implications and Applications
This study suggests that the slashing technique is more suitable for harvesting a
greater number and superior quality of COC. The culture conditions used in this experiment
were found to be optimal and efficiently supported in vitro maturation, IVF, and subsequent
development of goat embryos to the blastocyst stage. Based on the results of this
study, the culture conditions and slashing technique could be used in caprine in vitro
embryo production and advanced biotechnology research.
Key words:
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Article Info
Publication History
Accepted:
July 23,
2022
Received:
March 1,
2022
Footnotes
The authors have not declared any conflicts of interest.
Identification
Copyright
© 2022 American Registry of Professional Animal Scientists. Published by Elsevier Inc. All rights reserved.
