Advertisement

Effects of whole cottonseed supplementation on performance, semen quality, and manganese superoxide dismutase concentrations in blood and semen of beef bulls

      This paper is only available as a PDF. To read, Please Download here.

      ABSTRACT

      Objective

      This research aimed to determine the effects of whole cottonseed on performance, semen quality, and manganese superoxide dismutase (MnSOD) concentrations in the blood and semen of beef bulls.

      Materials and Methods

      In a completely randomized design, 46 (BW = 457 ± 12 kg; 16–18 mo old) Angus bulls were stratified by BW and randomly assigned to 1 of 3 treatments: DD (n = 14, control; 3.18 kg/d dried distillers grain), WD [n = 16; 1.59 kg/d whole cottonseed (0.56 and 0.82% free gossypol in yr 1 and 2, respectively) and 1.59 kg/d dried distillers grain], or WW (n = 16; 3.18 kg/d whole cottonseed). Bulls were weighed and blood and semen were sampled on d 0, 30, and 60. Body weights and morphological characteristics of spermatozoa were measured in both years, and MnSOD concentrations of blood and semen were measured in yr 1.

      Results and Discussion

      There was a treatment effect for ADG (P = 0.002) as bulls fed WW had a decreased (P < 0.03) ADG compared with bulls fed WD and DD. A time effect was observed for scrotal circumference (P < 0.001). Scrotal circumference measurements increased on d 60 compared with d 0 and 28 (P ≤ 0.02). There was no time main effect for percentage of spermatozoa with other abnormalities (P = 0.87); however, there was a treatment effect (P = 0.04). Bulls in the WW treatment had fewer other abnormalities than bulls in DD (P = 0.01) but were not different than WD (P = 0.27).

      Implications and Applications

      Whole cottonseed did not affect reproductive parameters measured in this study or blood and semen concentrations of MnSOD. According to these results, WCS supplemented to beef bulls at 0.7% of BW for 60 d may not negatively affect semen quality or MnSOD concentrations in the blood or semen.

      Key words

      LITERATURE CITED

        • Agarwal A.
        • Makker K.
        • Sharma R.
        Clinical relevance of oxidative stress in male factor infertility: An update.
        Am. J. Reprod. Immunol. 2008; 59: 2-11https://doi.org/10.1111/j.1600-0897.2007.00559.x
        • Arshami J.
        • Ruttle J.L.
        Effects of diets containing gossypol on spermatogenic tissues of young bulls.
        Theriogenology. 1988; 30: 507-516https://doi.org/10.1016/0093-691X(88)90200-2
        • Bansal A.K.
        • Bilaspuri G.
        Impacts of oxidative stress and antioxidants on semen functions.
        Vet. Med. Int. 2010; 2011: 1-7https://doi.org/10.4061/2011/686137
        • Bertrand J.A.
        • Sudduth T.Q.
        • Condon A.
        • Jenkins T.C.
        • Calhoun M.C.
        Nutrient content of whole cottonseed.
        J. Dairy Sci. 2005; 88: 1470-1477https://doi.org/10.3168/jds.S0022-0302(05)72815-0
        • Calhoun M.
        • Huston J.
        • Kuhlmann S.
        • Baldwin B.
        • Engdahl B.
        • Bales K.
        Comparative toxicity of gossypol acetic acid and free gossypol in cottonseed meal and Pima cottonseed to lambs. Page 4779 in Progress Report.
        Agric. Exp. Stn, Texas1990
        • Calhoun M.
        • Huston J.
        • Kuhlmann S.
        • Baldwin B.
        • Menges W.
        • Engdahl B.S.
        • Ueckert D.N.
        • Engdahl G.R.
        Increased erythrocyte fragility in cattle, sheep, and goats fed whole cottonseed. Page 4780 in Progress Report.
        Agric. Exp. Stn, Texas1990
        • Chase Jr., C.C.
        • Bastidas P.
        • Ruttle J.L.
        • Long C.R.
        • Randel R.D.
        Growth and reproductive development in Brahman bulls fed diets containing gossypol.
        J. Anim. Sci. 1994; 72: 445-452https://doi.org/10.2527/1994.722445x
        • Chenoweth P.J.
        • Chase Jr., C.
        • Risco C.
        • Larsen R.
        Characterization of gossypol-induced sperm abnormalities in bulls.
        Theriogenology. 2000; 53: 1193-1203https://doi.org/10.1016/S0093-691X(00)00264-8
        • Cusack P.M.V.
        • Perry V.
        The effect of feeding whole cottonseed on the fertility of bulls.
        Aust. Vet. J. 1995; 72: 463-466
        • Gadelha I.C.
        • Fonseca N.B.
        • Oloris S.C.
        • Melo M.M.
        • Soto-Blanco B.
        Gossypol toxicity from cottonseed products.
        Scientific- WorldJournal. 2014; 2014: 1-11https://doi.org/10.1155/2014/231635
        • Haschek W.
        • Beasley V.
        • Buck W.
        • Finnell J.
        Cottonseed meal (gossypol) toxicosis in a swine herd.
        J. Am. Vet. Med. Assoc. 1989; 195: 613-615
        • Hill G.M.
        • Meyer J.E.
        • Fritsche K.L.
        Effect of vitamin E and selenium on iron utilization in neonatal pigs.
        J. Anim. Sci. 1999; 77: 1762-1768https://doi.org/10.2527/1999.7771762x
        • Jeeva J.S.
        • Sunitha J.
        • Ananthalakshmi R.
        • Rajkumari S.
        • Ramesh M.
        • Krishnan R.
        Enzymatic antioxidants and its role in oral diseases.
        J. Pharm. Bioallied Sci. 2015; 7: 331-333https://doi.org/10.4103/0975-7406.163438
        • Jimenez D.
        • Chandler J.
        • Adkinson R.
        • Nipper W.
        • Baham A.
        • Saxton A.
        Effect of feeding gossypol in cottonseed meal on growth, semen quality, and spermatogenesis of yearling Holstein bulls.
        J. Dairy Sci. 1989; 72: 1866-1875https://doi.org/10.3168/jds.S0022-0302(89)79305-X
        • Johnson M.A.
        • Murphy C.L.
        Adverse effects of high dietary iron and ascorbic acid on copper status in copper-deficient and copper-adequate rats.
        Am. J. Clin. Nutr. 1988; 47: 96-101https://doi.org/10.1093/ajcn/47.1.96
        • Koziol J.H.
        • Armstrong C.L.
        Manual for Breeding Soundness Examination of Bulls.
        2nd ed. Soc. Theriogenol, 2018
        • Lowry O.H.
        • Rosebrough N.J.
        • Farr A.L.
        • Randall R.J.
        Protein measurement with the Folin phenol reagent.
        J. Biol. Chem. 1951; 193: 265-275
        • Marklund S.
        • Marklund G.
        Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase.
        Eur. J. Biochem. 1974; 47: 469-474https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
        • Moore J.A.
        • Swingle R.S.
        • Hale W.H.
        Effects of whole cottonseed, cottonseed oil or animal fat on digestibility of wheat straw diets by steers.
        J. Anim. Sci. 1986; 63: 1267-1273https://doi.org/10.2527/jas1986.6341267x
        • Morgan S.
        • Stair E.
        • Martin T.
        • Edwards W.
        • Morgan G.
        Clinical, clinicopathologic, pathologic, and toxicologic alterations associated with gossypol toxicosis in feeder lambs.
        Am. J. Vet. Res. 1988; 49: 493-499
        • NASEM (National Academies of Sciences, Engineering, and Medicine)
        Nutrient Requirements of Beef Cattle.
        8th ed. Natl. Acad. Press, 2016https://doi.org/10.17226/19014
        • Nichi M.
        • Bols P.E.
        • Züge R.M.
        • Barnabe V.H.
        • Goovaerts I.G.
        • Barnabe R.C.
        • Cortada C.N.
        Seasonal variation in semen quality in Bos indicus and Bos taurus bulls raised under tropical conditions.
        Theriogenology. 2006; 66: 822-828https://doi.org/10.1016/j.theriogenology.2006.01.056
        • Prasad M.R.
        • Diczfalusy E.
        Gossypol.
        Int. J. Androl. 1982; 5: 53-70https://doi.org/10.1111/j.1365-2605.1982.tb00304.x
        • Risco C.A.
        • Chenoweth P.
        • Larsen R.
        • Velez J.
        • Shaw N.
        • Tran T.
        • Chase Jr., C.
        The effect of gossypol in cottonseed meal on performance and on hematological and semen traits in postpubertal brahman bulls.
        Theriogenology. 1993; 40: 629-642https://doi.org/10.1016/0093-691X(93)90415-2
        • Smith W.A.
        • Vosloo L.P.
        • Van Niekerk C.H.
        • Theron F.P.
        Effect of free gossypol in whole cottonseed on the semen quality of Holstein bulls.
        S. Afr. J. Anim. Sci. 1991; 21: 16-20
        • Stewart Jr., R.L.
        Using Cotton Byproducts in Beef Cattle Diets.
        B1310. Univ. Georgia, Athens2010
        • Velasquez-Pereira J.
        • Chenoweth P.
        • McDowell L.
        • Risco C.
        • Staples C.
        • Prichard D.
        • Martin F.
        • Calhoun M.
        • Williams S.
        • Wilkinson N.J.
        Reproductive effects of feeding gossypol and vitamin E to bulls.
        J. Anim. Sci. 1998; 76: 2894-2904https://doi.org/10.2527/1998.76112894x
        • Vince S.
        • Žura Žaja I.
        • Samardžija M.
        • Majić Balić I.
        • Vilić M.
        • Đuričić D.
        • Valpotic H.
        • Markovic F.
        • Milinković-Tur S.
        Age- related differences of semen quality, seminal plasma, and spermatozoa antioxidative and oxidative stress variables in bulls during cold and warm periods of the year.
        Animal. 2018; 12: 559-568https://doi.org/10.1017/S1751731117001811
        • Wang X.
        • Howell C.P.
        • Chen F.
        • Yin J.
        • Jiang Y.
        Gossypol—A polyphenolic compound from cotton plant.
        Adv. Food Nutr. Res. 2009; 58: 215-263https://doi.org/10.1016/S1043-4526(09)58006-0